Hifiscript cdna

Author: aqkh

August undefined, 2024

WebThe kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Remover, the sample … WebDescripción. The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is …

康为世纪 - Cwbio

Web6 de ago. de 2024 · The biological function of the aromatase gene (cyp19a) is to transform testosterone into estradiol, which is involved in ovarian differentiation and development. In the present study, in order to explore the role of cyp19a in sex differentiation of Culter alburnus, the full-length cDNA sequence was obtained, and the relationship between … cannot find dispatch method

Genome‐wide lncRNAs identification and association analysis for …

Web15 de set. de 2024 · R6834, Omega bio-tek, Norcross, GA, USA) and reversely transcribed using a HiFiScript cDNA Synthesis Kit (Cat. No. CW2569, CWBIO, Taizhou, China). Reverse transcription was performed at 42 C for 15 min, followed by 85 C for 5 min to inactivate the enzyme activity. Samples were subjected to qPCR using a Thermoﬁsher … Web11 de jan. de 2015 · 按HiFiScript 快速去基因组cDNA 第一链合成试剂盒说明将RNA 逆转录成cDNA，按2×Taq Master Mix说明进行扩增。PCR 扩增反应体系:cDNA 1 μL、上游和下游引物各1 μL、2×Master Mix 12.5 μL，ddH2O 9.5 μL，总反应体系25 μL。扩增后产物用1%琼脂糖凝胶电泳25 min 左右。 WebFirst-stranded cDNA was synthesized from 4 μg RNA using a HiFiScript cDNA Synthesis kit (YEASEN, Shanghai, China) and applied as a template for qRT-PCR. Dissociation curves of qRT-PCR reaction were analyzed for the specificity of primers. qRT-PCR analysis was run on CFX96 Real Time PCR System (Bio-rad, America) using UltraSYBR mixture … f j p winners list

HiFiScript gDNA Removal cDNA Synthesis Kit

Web24 de mai. de 2024 · Ultrapure RNA kits (CW0581M), HiFiScript cDNA synthesis kits (CW2569M), and UltraSYBR mixture (CW0957M) were purchased from CWBIO (Beijing, China). Human A549 lung carcinoma cells were provided by Stem Cell Bank, Chinese Academy of Sciences, Shanghai, China. Web2）rnaseh活性：在反应过程中切割rna：cdna杂合链中的rna模板。降低此活性，可避免在合成较长cdna之前模板rna被降解掉。 3）dna指导的dna聚合酶活性：以反转录合成的第 … fjr1300 accessories ebayWebOneScript® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the ... fjqss_collect

"WebThis product is a kit for reverse transcription after removal of genomic DNA. The kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription … " - Hifiscript cdna

Hifiscript cdna

High Capacity RNA-to-cDNA Products - Thermo Fisher Scientific

Web19 de jul. de 2024 · Subsequently, the total RNA was reverse transcribed into cDNA with the use of HiFiscript cDNA Synthesis Kit according to the manufacturer’s instructions. Quantitative real-time PCR was performed using 2× Fast Super EvaGreen® qPCR Mastermix on a LightCycler96 Real-Time PCR system (Roche, Basel, Switzerland). WebHifiscript 1st Strand cDNA Synthesis Kit. Catalog number: 101Bio-W2569-100. Size: 100 Reactions. Price: 769.5 USD. Hifiscript 1st Strand cDNA Synthesis Kit. Catalog number: 101Bio-W2569-25. Size: 25 Reactions. Price: 228 USD. HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper), 50 tests (20 μl/tests)

Did you know?

Web18 de jul. de 2024 · HiFiScript gDNA Removal cDNA Synthesis Kit Catalog Number: CW2024S (10 rxns) CW2024M (100 rxns) Storage Condition:-20°C . Kit Components: … Web18 de jul. de 2024 · HiFiScript gDNA Removal cDNA Synthesis Kit Catalog Number: CW2024S (10 rxns) CW2024M (100 rxns) Storage Condition:-20°C . Kit Components: Component CW2024S (10 rxns) CW2024M (100 rxns) gDNA Remover Mix 10 µl 100 µl 5×HiFiScript RT MasterMix 40 µl 400 µl RNase-Free Water 0.5 ml 1.5 ml

WebWhen the cells grew to 80% confluence, they were cultured for 48 h in media containing different concentrations of glucose: 0 (Control), 5.5, 15 and 25 mmol/l. Total RNA from multiple cells was extracted using TRIzol reagent and reverse transcribed into cDNA using the HiFiScript cDNA synthesis kit, according to the manufacturer's protocol. WebHiFiScript cDNA Synthesis Kit. This product is a first-strand cDNA synthesis kit designed for the first step of two-step RT-PCR. The HiFiScript reverse transcriptase used in the …

WebHiFiScript gDNA Removal RT MasterMix GoldStar Probe mixture HiFiScript cDNA Synthesis Kit HiFiScript gDNA Removal cDNA Synthesis Kit HiFi PCR Mix for NGS MagicSYBR Mixture SARS-CoV-2 Detection Kit (qPCR Probe Assays) Bst DNA Polymerase, Large Fragment Cobuddy DNA Polymerase UltraSYBR One Step RT-qPCR … WebThis product is a kit for reverse transcription after removal of genomic DNA. The kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Eraser, the sample processed by gDNA Eraser can be directly used for reverse transcription reaction to synthesize cDNA.This ...

Web1 de fev. de 2024 · First-strand cDNA was synthesized using ProtoScript II Reverse Transcriptase and the second-strand cDNA was synthesized using Second Strand Synthesis Enzyme Mix. The bead-purified double-stranded cDNA was then treated with End Prep Enzyme Mix to repair both ends and add a dA-tailing in one reaction, followed by a …

WebThe High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high quality first-strand cDNA from total RNA. The kit was … fjr1300as 20thWebHappy Scribe lets you choose between automatically transcribing your files or using our human transcription service. Our transcription software uses speech recognition … cannot find efi directory arch linuxWebFeatures. Rapid genomic DNA deletion: With the gDNA remover, it takes only 2 minutes to remove genomic DNA. Rapid reverse transcription: It takes only 15 minutes to obtain the … cannot find either column or user definedWeb16 de jul. de 2024 · HiFiScript gDNA Removal cDNA Synthesis Kit Catalog Number: CW2582S (25 rxns) CW2582M (100 rxns) Storage Condition:-20°C. Kit Components: … cannot find discord toasterWeb[NEW Products] Endotoxin-Free Plasmid miniprep Link... cannot find downloaded filesWebtranscription, by applying the HiFiScript cDNA first-strand synthesis kit (CW2569M; CWBIO). We used fluorescent PCR instrument (CFX Connect™ real-time; Bio-Rad Laboratories) for performing RT-qPCR. The reaction system was: RNase Free dH2O 9.5 μL; cDNA 1 μL; upstream primer 1 μL; downstream primer 1 μL; and 2×qPCR mixture 12.5 μL. can not find dns api hook for: dns_dynuWeb8 de set. de 2024 · Subsequently, HiFiScript cDNA Synthesis kit (catalog no. CW2569; CWBiotech Co. Ltd.) was used to reverse transcribe 1 µg total RNA at 37°C for 15 min and 85°C for 5 sec into cDNA. qPCR was performed using a Mastercycler ep realplex cycler with SYBR ® Green Master Mix (catalog no. CW0957 fjr1300 clutch slave cylinder