Phospho p53bp1 fluorescent staining
WebJul 30, 2024 · We found that 53BP1 is already significantly phosphorylated at mild dose of HU ( Fig 1C) and accumulates in foci in replicating cells only 30 minutes after induction of DRS ( S1B and S1C Fig ). Importantly, because we extracted soluble proteins before cell fixation, we only quantified proteins on chromatin and therefore DSB bound p53BP1. WebIdentify the problem with your immunofluorescence staining from the options below: Weak or No Staining High Background Non-specific Staining Weak or No Staining Incorrect light source/filter set: Ensure your microscope is equipped with the correct light source and filter set for the fluorophore you have chosen. Gain/exposure is too low:
Phospho p53bp1 fluorescent staining
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WebBioss Anti-Phospho-p53BP1 (Ser25, Ser29) Polyclonal, Catalog # BS-3020R. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Frozen) (IHC (F)), … WebNov 7, 2024 · P53BP1 is a well-characterized P53 cofactor, and the contribution of P53BP1 to transcriptional regulation of the DNA damage checkpoint has been reported to be P53-dependent yet its direct role in ...
WebThe p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and … WebSep 17, 2003 · In order to optimize staining levels, phospho-specific mAbs were titrated on unstimulated and stimulated samples to find the concentration that maximized the fold …
Web53BP1 was originally identified as a binding partner for p53, and suggested to enhance its transcriptional activity 6, 7. 53BP1 plays an essential role in DNA repair; it is known to be recruited to sites of DNA damage, and retention of 53BP1 at these sites is dependent on gamma-H2A.X 8. WebStaining Surface Molecules: Prepare a cocktail of fluorescent antibodies specific for staining cell surface antigens in 1× TFP Perm/Wash Buffer or Stain Buffer (BSA). For example, dilute fluorescent surface antibodies at a 10 µL:1000 µL dilution to make a cocktail of 50 tests at 20 µL per test. 14. Incubate for 40-50 min at 2-8ºC . 15.
Webphospho-specific; control iggs and igg conjugates; control sera for ihc staining; monoclonal mouse anti-rabbit/goat secondary antibodies; polyclonal goat/rabbit anti-mouse …
WebResuspend the cells in Stain Buffer (FBS) at 10 million cells/mL and aliquot 100 μL to each sample tube. Aliquot an optimal concentration of phosphospecific fluorochrome … cancer drug extends lifespanWebJun 22, 2001 · Whereas acetylated at G 0, p53 was found to exhibit phosphorylation on residues 15, 20, and 372 at the G 1 phase of the cell cycle and on aa 37 and 392 during the … cancer drug list mohWebWestern blot analysis of extracts from Jurkat cells, untreated (-) or treated with LY294002 #9901 or Calyculin A #9902 (+), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060 (upper), Akt (pan) (C67E7) Rabbit mAb #4691 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). The western blot membranes were treated with our standard antibody diluent and … fishing temperatureWebThis antiserum recognizes 53BP1 only when both Thr1609/Ser1618 residues are phosphorylated, but not either one alone (PMID 24703952). The sequence surrounding … cancer drugs covered by part bWebJan 10, 2024 · We describe a standard protocol for phosphate-affinity fluorescent gel staining that uses a fluorophore-labeled dizinc (II) complex of a derivative of the … cancer drugs medishieldWebPhospho-53BP1 (Thr543) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan ®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Thr543 was discovered using an ATM/ATR substrate antibody and was shown to be induced by UV treatment. cancer drug cytoxanWebPhospho-53BP1 (Thr543) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan ®, CST's LC-MS/MS platform for modification site … fishing television